- "I'm going to save the human race, Sara, maybe you can help me."
- —Ivo offering Sara a position in his field of work[src]
Dr. Anthony Ivo was a scientist looking to unearth a long-lost medicinal and chemical breakthrough dating back to the later days of World War II named Mirakuru, a deadly serum that gave those who consumed it superhuman abilities. He was killed by Oliver Queen after a series of conflicts on Lian Yu, the serum's final resting place.
In "League of Assassins", when Sara Lance awoke on the Amazo after almost drowning, she found herself in a cage. Anthony ordered "The Captain" to release her, and he took her back to his quarters. He informed her that he was a scientist who intended to "save the human race".
In "Keep Your Enemies Closer", quite a while later, after capturing Oliver Queen (who adopted the pseudonym "Tommy"), he interrogated him, explaining that he was looking for a sunken submarine containing a serum created by the Japanese army during World War II, Mirakuru. After Sara tricked Oliver into confirming that his friends were alive, Anthony declared that Oliver would take them to Slade and Shado on Lian Yu, and then revealed that he was aware that his real name was actually Oliver.
In "State v. Queen", Ivo and his men took Oliver and Sara to land. They went to find Slade and Shado in their hideout, but when they couldn't find them, he ordered "The Captain" to blow the hideout up. He forced Oliver to take him to the grave of the Japanese soldiers that Oliver had stumbled on earlier, and when they arrived, he and his men searched for the hōzen, which Oliver had previously taken. When they were unable to find it, Anthony grew angry and demanded that Oliver tell him where it was, having "The Captain" threaten to shoot Oliver's hand off. After Slade and Shado ambushed Anthony's group, he agreed to release Oliver. After the others escaped safely, Anthony shot an already-wounded Captain in the head for his failure to prevent Oliver's escape, and promoting the first mate of the Amazo to be the new captain.
In "The Scientist", Ivo and his crew followed Oliver, Shado, Sara and a wounded Slade to the submarine. After they injected Slade with a dose of raw Mirakuru, Ivo and his men burst in, holding the three of them at gunpoint.
In "Three Ghosts", Ivo took a defenseless Oliver, Shado and Sara to the surrounding forest. He forced Oliver to decide which one of the two women would be killed, threatening that if Oliver did not choose, he would kill both of them. When Oliver leaped to try and stop him, Ivo killed Shado. A Mirakuru-enhanced Slade then burst out of the forest and wiped out most of his men, forcing Ivo to flee.
In "Blast Radius", when Sara, Oliver and Slade all escaped Ivo, they set up camp for the night. Ivo contacted them using a walkie-talkie, telling Sara that he would ravage the whole island if it meant he could get the Mirakuru back. The group did not respond.
In "Blind Spot ",
In "The Promise", it is revealed that Ivo's wife Jessica has developed severe mental illness; it is implied that he is researching Mirakuru in hopes of finding a cure for her. As part of his plan to take the Amazo, Oliver allowed himself to be captured and interrogated by Ivo.
After being cornered by Oliver on a subsequent occasion, Ivo lures him into discussing Shado's death so that a nearby Slade can overhear. Slade later captures Ivo after taking over the Amazo. After he orders Ivo to reenact Shado's murder, Slade cuts off Ivo's right hand.
In "The Man Under the Hood", Ivo is slowly dying from gangrene resulting from his amputated hand. He tells Sara and Oliver that he created a cure to counteract the effects of the Mirakuru; in return for the cure's location, he requests a quick death. They agree, and Ivo tells them that the cure is in the Amazo and gives them the key to it. Then Sara points a pistol at Ivo and is about to kill him, when Oliver stops her and kills Ivo himself with two shots to the chest.
It can be assumed that Ivo was once a good man who was passionate about his work and dearly loved his wife, Jessica. However, once she became ill, it transformed Anthony into a murderous and obsessed man. He wanted to locate Mirakuru, a Japanese super soldier drug that grants someone an accelerated healing factor so that he could save his wife. In pursuit of his goals however Ivo was cold, ruthless, amoral and utterly uncaring; he had imprisoned countless people and relentlessly experimented on them for science. He also made one of his crew members the Captain shoot his captives, so that they may remove the bullet and prove they are strong, Ivo's true cruelty had never been highlighted until he had finally located the Mirakuru and forced Oliver to decide who he should spare between Sara and Shado before executing Shado in cold-blood.
As a scientist Ivo is very manipulative, he realized that Slade had loved Shado and got Oliver to admit that her death was his fault, which prompted his former friend to attack Oliver.
Ivo is somewhat predictable, Sara realized that when Oliver was captured on board the Amazo he would use truth serum to get him to talk which was correct and it led his men into a trap in the boiler room.
He cared very little for his crew, as shown when he shot the Captain out of frustration for him constantly failing Ivo and then promoted the Butcher in his place but despite this, he showed genuine concern for Sara Lance and saved her from being one of his science experiments, she also apparently had a place on Ivo's table as she was able to stop the Captain from killing Oliver but after Sara helped him, Shado and Slade Anthony began to show less concern for her wellbeing and when she rebuffed his offer for a way off of Lian Yu for the Mirakuru he lost all loyalty towards Sara and rudely calls her an "ungrateful bitch."
Despite all of his wicked deeds when his hand was lacerated by Slade Wilson, he began to die and had the audacity to ask for a quick death, something which he did not deserve.
- "League of Assassins" (flashback)
- "Keep Your Enemies Closer" (flashback)
- "State v. Queen" (flashback)
- "The Scientist" (flashback)
- "Three Ghosts" (flashback)
- "Blast Radius" (voice)
- "Blind Spot" (flashback)
- "The Promise" (flashback)
- "Deathstroke" (flashback)
- "The Man Under the Hood" (flashback)
- "Public Enemy" (flashback)
Behind the scenes
- In the DC comics, Anthony Ivo, known as Professor Ivo, is a cybernetics genius who created Amazo, the synthetic android with the capacity to duplicate the powers of all the Justice League members.
- Ivo's ship is called the Amazo as a reference to the android he created in his comic book incarnation.